Neuroprotective Mechanism of Corydaline in Glutamate-Induced Neurotoxicity in HT22 Cells
Baskar Selvaraj, 김대원, 유기연, 박근완, Thi Thu Thuy Tran, 이재욱, 이희수,
소속 상세정보
( Baskar Selvaraj ) -
김대원 ( Kim Dae-Won ) -
유기연 ( Yoo Ki-Yeon ) -
박근완 ( Park Keun-Wan ) -
( Thi Thu Thuy Tran ) -
이재욱 ( Lee Jae-Wook ) -
이희수 ( Lee Hee-Su ) -
Abstract
Glutamate-mediated oxidative stress causes neuronal cell death by increasing intracellular Ca 2+ uptake, reactive oxidative species (ROS) generation, mitogen-activated protein kinase (MAPK) activation, and translocation of apoptosis-inducing factor (AIF) to the nucleus. In the current study, we demonstrated that corydaline exerts potent neuroprotective effects against glutamate-induced neurotoxicity. Treatment with 5 mmol/L glutamate increased cellular Ca 2+ influx, ROS generation, MAPK activation, and AIF translocation. In contrast, corydaline treatment decreased cellular Ca 2+ influx and ROS generation. Western blot analysis revealed that glutamate-mediated MAPK activation was attenuated by corydaline treatment. We further demonstrated that corydaline treatment inhibited the glutamate-mediated translocation of AIF to the nucleus. We propose that corydaline is a promising lead structure for the development of safe and effective neuroprotectants.
키워드
Glutamate-induced neurotoxicity; HT22 mouse hippocampal neuronal cells; Corydaline
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